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However, with the addition of medium length PEG-2000 polymer chain, most of the large flower-like hierarchical architectures disappeared and the well-defined hollow architectures were formed.
Trypsinization was stopped by the addition of medium containing 10% FBS (without antibiotics).
Trypsinization was stopped by the addition of medium containing 10% FBS.
Following the addition of medium, the vials were sealed using silicone stoppers and aluminum crimp seals (Supelco), and autoclaved.
The plates were incubated at 37°C for 30 min, allowing gel polymerization prior to addition of medium.
The addition of medium, conditioned with active Sulf-2 protein, did not promote Wnt signaling over the basal level.
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Addition of medium-MW EHBPES-2 not only significantly improves toughness and elongation at break but also increases tensile strength and Tg.
In addition, artists often modified their paints through the mixing of colors, as well as the addition of mediums, thinners, driers, waxes etc. to customize their handling and optical properties.
Cells were washed once with 180 μl serum-free medium, before addition of new medium with or without test substances.
The treated cells were washed once with culture medium, and after addition of fresh medium incubated at 37°C before further analysis.
Subsequently, medium was removed and the cells were washed twice with medium, followed by the addition of fresh medium.
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