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The value for E for the dimeric form can also be obtained by addition of fragments, as we have done for cinnamic acid monomer.
At 10% cut-off, the number of these fragments already reaches half the sample size (due to addition of fragments 43u, 48nu, 62nu).
Figures showing the chemical shift perturbations of the PRDX5 protein upon addition of fragments, identification of the binding region for the 6 hit fragments, superposition of the docked structure of fragment F152 with the X-ray structure of the benzoate, and the plot of the dose-response relationship of the inhibition of PRDX5 by F152.
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In contrast, when VEGFf binding inhibits interactions between VEGFR1 and NP-1 (Model 2), addition of fragment more than doubled the level of VEGF retained by VEGFR2.
Concomitantly, Myrmel et al. provided concrete evidence demonstrating that addition of fragment 2 to α-thrombin increases significantly the catalytic efficiency of the enzyme toward TAME, compared to the efficiency of α-thrombin alone against the same substrate (28).
In addition, amplification of fragments from two X-linked and two autosomal scaffolds as identified by the AD-ratio approach was made in both male and female bears to verify their non-Y-chromosomal origin.
Addition of some fragments resulted in a decrease of RU, suggesting that they inhibited the Tob-CNOT7 interaction (Fig. 1F).
This experiment tests the impact of the addition of larger fragments that sample the correlation between multiple torsion angles.
In addition, studies have detected transgene rearrangements, such as sequence deletions [13 15] and nucleotide addition of undesired fragments into the transgene sequence [16], and also, alterations in mRNA expression levels were also observed [17].
Addition of fibronectin fragments to these other matrices was sufficient to elicit the invasive phenotype [ 10].
Moreover, the addition of fibronectin fragments reversed the reduction of cell invasion observed following PIP-knockdown to approximately 80% of the control levels.
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