Sentence examples for adding to the reaction from inspiring English sources

The RT was either incubated with 25 μM aptamer or without aptamer for 15 min at room temperature (20 °C), before adding to the reaction mix.

The reverse transcriptase was incubated with different concentrations of the aptamer (0, 12.5, 25, 50, 100, and 200 μM per reaction) before adding to the reaction mix.

The reverse transcriptase was incubated with increasing concentrations (0, 12.5, 25, 50, 100, or 200 μM) of aptamer for 15 min at room temperature (20 °C) before adding to the reaction mix.

In order not to alter the surface charge of the particles, fluorescein isothiocyanate (FITC -modified aminopropyltriethoxysilane silane (APTES) was mixed with the silica source beFITC -modified the reaminopropyltriethoxysilane co-condensilanenctionAPTEStion of FITC washin the silica framixedk.

Prior to setting up RT-PCR experiments, RNA was incubated for 5 minutes at 95°C and cooled on ice for at least one minute before adding to the reaction.

Various substrates were added to the reaction mixture.

Then, the ACVA solution was added to the reaction mixture.

Then trimethylsilylimidazole (1.5 mL) was added to the reaction mixture.

was added to the reaction followed by H2O (2 mL).

Solution B is then added to the reaction mixture, and water is immediately added.

This solution was then added to the reaction vessel with the temperature raised to 70°C.