Sentence examples for adding the substrate from inspiring English sources

Finally, after adding the substrate, absorbance at 405 nm was read.

Before adding the substrate (d-xylose or d-xylonate) to the reactions, the background formation of NADH was monitored and subtracted, if present.

The protoplasts showed low RLU (about 20 RLU) soon after adding the substrate, suggesting interaction did not occur at this point.

RLU increased soon after adding GA3 and GA4, and the RLU remained at high levels even 30 min after adding GA3 and GA4 (90 min after adding the substrate).

On the other hand, the RLU increased soon after adding GA3, and the RLU remained at high levels even 30 min after adding GA3 (90 min after adding the substrate).

The effect of metallic ions at concentration of 5 mM, chelating agents, surfactants, and inhibitors on the activity of crude xylanase were determined by preincubating the enzyme in the presence of Na+, Mg2+, Ca2+, Mn2+, Fe2+, Zn2+, Cu2+, K +, Hg2+, and Cd2+, EDTA (5 Mm), SDS (1%), β-mercaptoethanol (20 mM), and Triton X-100 (1%) for 30 min at 55 °C before adding the substrate (Ozcan et al. 2011).

As the binding of these inhibitors is state-dependent, it was necessary to also add the substrate ADP to allow cycling between states.

Add the substrate and/or airstones.

Add the substrate, rocks, decorations, filters, heaters, etc. back and plug them in and turn them on.

Before adding the gravel, fill the tank up with tap water, to the depth of how high you want the gravel, then add the substrate.

Add the substrate to the tank about 2 to 3 inches (5.1 to 7.6 cm) thick or 1.5 2.5 inches (3.8 6.4 cm) of dirt capped with 0.5 inches (1.3 cm) of sand.