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Only Ligand was added in place of complex to prepare the Non-IIP by the discussed method, the rest of the procedure is same.
The results revealed that the pH value change after the acidizing is not much when organic mud acid is used as compared to standard mud acid, and this change is even less when fluoboric acid was added in place of hydrofluoric acid.
He added: "In place of the casino economy, one where people who work hard can actually get on; in place of the welfare society, one where no individual is written off; in place of the broken education system, one that gives every child the chance to rise up and succeed".
On both ships, two more quadruple sets of 1.1 in guns were added in place of two searchlights amidships.
A high-angle director for the anti-aircraft guns was also added in place of the aft rangefinder.
Assay B: Assay B followed the identical procedure, except the deuterated aldehyde 4-formylphenoxypropyl triphenylphosphonium-d15 bromide [11] was added in place of the non-deuterated aldehyde.
Reactions were incubated 10 min at room temperature, 42°C for 50 min and 70°C for 15 min. For oligo dT) priming, 250 ng oligo dT 18 was added in place of hexamers, and reactions were heated to 42°C prior to enzyme addition.
All reactions utilized a control in which PBS was added in place of the probe.
For the controls, 10 μL of pure DMSO was added in place of the inhibitor solution.
1 μL of RNase-free water was added in place of RT.
Extraction from an empty gel was performed and this solution was added in place of enzyme solutions for the control.
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