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That's normally done by adding a cocktail of six enzymes to a solution containing the plants.
Non-specific proteolysis that could remove the protein signal was prevented by adding a cocktail of protease inhibitors to the buffers.
Slides were prepared for 4°C overnight incubation (12 hours) by adding a cocktail of MAP-Tau primary antibody (1 750) plus a wide-spectrum rabbit anti-cow cytokeratin antibody (Z0622; DAKO, Carpinteria, CA) diluted 1 100 in BSA/1X TBS-T.
Molecular weight standards were generated by adding a cocktail of antibodies to P190 (190 kD), Adaptin beta (106 kD), STAT-3 (92 kD), PTP1D (72 kD), Mek-2 (46 kD), RACK-1 (36 kD), GRB-2 (24 kD) and Rap2 (21 kD) to lane 40 of gels A-D.
Molecular standards for gel E were generated by adding a cocktail of antibodies to Exportin-1/CRM1 (112 kD), MCM (83 kD), Nucleoporin p62 (62 kD), α-tubulin (55 kD), Actin (42 kD), KNP-1/HES1 (28 kD) and NTF2 (15 kD) to lane 16, and antibodies to p190 (190 kD), Hip1R (120 kD), Transportin (101 kD), Calreticulin (60 kD), Arp3 (50 kD), eIF-6 (27 kD) and Rap2 (21 kD) to lane 17.
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To do it, the industry must add a cocktail of expensive enzymes to break down the plants into sugars that microbes can easily transform into chemical precursors for gasoline, diesel, and jet fuel.
After 24 hours of incubation with the antigen add a cocktail of proinflammatory mediators to the cell suspension to induce DC maturation: 1000 U/ml TNF-α, 10 ng/ml IL-1β, 10 ng/ml IL-6 plus 1 μM PGE2.
After 24 hours add a cocktail of proinflammatory mediators to the cell suspension: 1000 U/ml TNF-α, 10 ng/ml IL-1β, 10 ng/ml IL-6 plus 1 μM PGE2.
After five days, immature DCs were collected, transferred to new plates, and induced to mature by adding a cocktail consisting of 150ng/ml IL-6, 5ng/ml TNF, and 5ng/ml IL-1β (R&D Systems) and 1 μg/ml PGE2 (Sigma).
Add a cocktail and/or pair of expensive sunglasses, if making your friends gnash their teeth really does it for you.
Umami, a sleek and chic restaurant that last year added a cocktail bar, is one of the few places in town to combine high-end drinks with food.
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