Sentence examples for added vigorously from inspiring English sources

Then, 10 mL of KNO3 sat. aq). was added, vigorously stirred for 30 min and the organic layer was extracted.

Later, he added: "I vigorously contest all sorts of things that appear in the Observer, but if I wanted to correct or qualify everything that appeared in the Observer with which I [disagree] they would have to print an extra 72-page supplement every week".

Then, a freshly prepared sodium borohydride aqueous solution (0.1 M, 5 mL) was added under vigorously stirring.

Three milliliters of the seeds solution was added while vigorously mechanical stirring, followed by the addition of 1.7 mL of 1%% AgNO3 solution.

QuEChERS salt kit (1.0 g of CH3COONa and 6.0 g of MgSO4) was added and vigorously shaken using vortex mixer for 6 min.

Fourteen ml of the extracting solution was added and vigorously shaken for 30 s, using an electrical shaker and immediately filtered into other previously prepared test tubes carrying funnels and Whatman # 42 filter papers.

A 10 g of the homogenized sample were placed into 50-mL Teflon centrifuge tube to which 10 mL of ethyl acetate was added and vigorously shaken for 1 min using a vortex mixer at maximum speed.

The mixtures were incubated at 20°C for 5 min. Chloroform (200 µl) was then added, mixed vigorously, and incubated for a further 3 min. The samples were centrifuged (12,000×g, 15 min, 4°C) and the aqueous phases transferred to fresh tubes, mixed with 0.5 volumes isopropanol and incubated at 20°C for 10 min to precipitate the RNAs.

After 5 minutes incubation at room temperature 200 μl of Chloroform was added and vigorously vortexed.

The solution was cooled down to 50°C, and methanol (500 mL) was added with vigorously stirring.

One millilitre of aqueous ammonia solution (14%%) was added and vigorously mixed before centrifugation with 2500 rpm for 5 min.