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One milliliter of hydroxyproline developer (β dimethylaminobenzaldehyde solution) was added to the samples and the standards.
The IC was added to the samples, co-extracted, and co-amplified.
Recoveries of Sb added to the samples were in the range 82 108%.
The recoveries of lead spikes added to the samples ranged from 93 to 102%.
Inocula of isolated microorganisms were added to the samples of swine manure.
After grinding, 300 μl Agencourt®Chloropure lysis buffer was added to the samples.
A mixture of 13C12-labelled internal standards was added to the samples before the extraction procedure.
Excess anhydrous methanol was immediately added to the samples to flocculate the PbE NCs.
Undecane (C11) was used as internal standard and was added to the samples before GC analysis.
Phenolphthalein was added to the samples and titrated against standard H2SO4 until the pink colour disappeared.
Then 0.2 mL 4 mg/mL ONPG was added to the samples.
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