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To avoid infinite values, a value of 1 was added to the normalized count value of each gene with zero value before log2 transformation.
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To simulate the noise effects, white Gaussian noise with increasing normalized variation was added to the original phantom images.
Then, the top 1,000 most-frequent GO terms in Swiss-Prot were added to the set of predicted GO terms and sorted by the normalized score.
The normalized cell extracts of 293T cells transfected with HCV E2 expression plasmids were added to the wells, and plates were incubated for 60 min at 37°C.
Identical amounts of normalized IxAC proteins from I. ricinus and control RaHBP2 were then added to the AP hemolysis assay in the presence of AH50 volumes of serum.
To test the normalized-hill β-adrenergic model, we simulated a simple experiment in which NE is added to the system and subsequently removed.
The beads were air-dried, and then 20 μl of water was added to elute the normalized libraries.
For the purpose of logarithmic scale representation, a number of 2 reads was added to each normalized miRNA number.
The mean (valid) CT for each gene was subtracted from the mean (valid) CT for the 5 reference genes, then 10 was added to give a normalized range of expression from 0-15, wheacheach unit reflects a 2-fold change in expression.
To normalize for PNLIPRP2 expression, this correction factor was added to quantile-normalized and log2-transformed TBL1 and PIK3CA expression data.
For each patient, results from the target lesions were added to get the RECIST diameter sum, the sum of VIU and the sum of normalized IU.
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