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From a practical point of view, testing data can always be added to the normalization at test time, data re-normalized and the classifiers re-trained on the training data alone.
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Central compensation mechanisms add to the normalization that is the result of those earlier, more peripheral mechanisms.
In the parallel normalization, a modified reporter gene (chloramphenicol resistance gene with same adaptor-primer sequences) was added to the cDNA population before normalization at a desired redundancy rate (e.g. 1.0%) as a control.
Oligo dT) primer was added to the reaction for further normalization with the endogenous control gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH; [ 44]).
For these spectra, ethanol in a 1 3 ratio was added to the aqueous solution, again for normalization of the SERS signal.
The 15 non-malignant brain sample data sets were next added to the entire dataset, and z-normalization and hierarchical clustering was repeated.
A Canny edge map of the filtered optical flow magnitude was generated and added to the original image edge map following normalization.
Their study did not find strong evidence for normalization to contribute to attentional modulation, even though for some neurons (∽25 30% depending on the model tested) the fits were significantly improved when a free parameter of power (equivalent to some form of normalization) was added to the model.
As different neurons show different amounts of normalization, a normalization scaling term α is added to the equation.
Fixed concentration of short fluorescent oligonucleotide control sequences ('spikes'), complementary to the control oligonucleotides on microarray, were added to the final 80 μl hybridization solution for normalization purposes.
In the parallel normalization, the reporter gene was added to the cDNA population as internal control at 1.0% redundant rate.
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