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Exact(8)
Sodium azide, 0.1%, and polymethylsulfonyl fluoride, 2 mM, were added to conditioned medium collected ∼65 hours post infection.
Perchloric acid (150 µl) was added to conditioned media (150 µl), which was then vortexed and shook for 10 min. The samples were then centrifuged (13,000 rpm) for 20 min and 240 µl of supernatant were transferred into an amber eppendorff tube.
Each was added to conditioned medium during cell migration assays.
Myostatin was added to conditioned medium at indicated final concentration prior to myoblasts incubation.
Neutralization antibodies against IGF-1 and its corresponding receptor were simultaneously added to conditioned medium in order to elucidate the protective role of IGF-1 secreted by ASCs.
The internal standard PGE2d4 (Cayman) at a concentration of 10 ng ml 1 was added to conditioned media of 3T3 cells isolated 24 h after the irradiation.
Similar(52)
Convergence of the inverse problem improves when adding diverse (nonreciprocal) drawdown series, but does not improve when reciprocal information is added to condition the flow model.
Exposure to talc in the course of carpet installation should be added to conditions that can cause pneumoconioses, specifically silicatosis of the lung.
When 17AAG or Methylene Blue [32] was added to the conditioned media there was a similar reduction in wound healing.
Penicillin G (100 U ml−1) and streptomycin sulphate (0.1 mg ml−1) were added to all conditioned media.
System Biosciences released a proprietary reagent named ExoQuick that can be added to serum, conditioned cell media, or urine and is claimed to precipitate the exosomes [ 32].
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