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Lysostaphin and RNase A were then added to concentrations of 50 µg ml−1 and 60 µg ml−1, respectively, and the mixture was incubated at 37°C for 30 minutes.
The antibiotics ampicillin (amp), kanamycin (kan), or tetracycline (tet) were added to concentrations of 150, 100, and 20 ug/ml respectively, when needed (Sigma-Aldrich, St . Louis MO.
For the Opi− screen, agarose was reduced to 1.2%, and Dox was added to concentrations noted in the sections to follow.
After 24 h ampicillin was added to a concentration of 25 μg/mL.
In these solutions, CYS was added to a concentration of 2.5 μM prior to the additions of zinc and sulfide.
0.5 mL urine was added to each concentration and the volume was adjusted by the appropriate solvent for each method.
TFE was added to a concentration of 50%.
A small amount of trypsin was added to facilitate concentration.
TbCl3 was added to a concentration of 100 mM and incubated for 2h.
An aliquot of 50 mM DTT solution was also added to final concentration of 5 mM.
Compounds were added to a concentration of 1 μM.
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CEO of Professional Science Editing for Scientists @ prosciediting.com