Sentence examples for added separately for from inspiring English sources

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Briefly, 10 ng of total RNA was reverse transcribed using MultiScribe™ Reverse Transcriptase and RT primers, added separately for each miRNA according to the supplier's instructions.

In the TNF- α-treated with ICA intervention group, exogenous TNF- α (10 ng/mL) and ICA (10−8 mol/L) were simultaneously added into cell culture solution for 36 hours, and then TNF- α (10 ng/mL) was added separately for 36 hours.

For growth experiments, the electron donors were added separately from the prepared stocks to the NBAF medium.

The experiments were performed on agar plates with cerulenin and TFL added separately and scored for no growth/active growth after incubation for 3 days.

Thereafter, 1 μg of purified FXII Enzyme Research LaboratoriesSwanseasea, UK) in 100 μL Tris buffer (50 mM Tris, 175 mM NaCl, 7.5 mM EDTA, pH 8.4) was added to the fibrin clots and incubated for an additional 10 minutes at 37°C: 100 μL of S-2302 (2.5 mM) and S-2238 (1 mM) (both from Haemochrom Diagnostica) was added separately and incubated for 5 minutes at 37°C.

To this, Cr, Mn, Fe, Co, Ni, Cu, and Zn nitrate solutions (3 wt.%) were added separately and stirred again for about 4 h.

Sterile D-glucose or D-xylose was added separately. a codon adapted for expression in S. cerevisiae.

Delivery characteristics were added separately to Model 2 (adjusted for maternal age) to evaluate the contribution of each characteristic to the ORs of SES for CS birth.

To this preparation, 0.5 mL of 200 µg/mL substrate (cefotaxime and ceftazidime) was added separately and incubated at room temperature for 30 minutes.

The media were sterilized at 121 °C for 20 min; methanol was added separately, and a glucose solution was added separately after filtration sterilization.