Sentence examples for added restriction from inspiring English sources

ccdB-ecoRI and ccdB-pstI primers represented added restriction sites for EcoRI and PstI enzymes (in bold) at their 5′ end with a couple of stabilising nucleotides, following New England BioLabs' guidelines (New-England-Biolabs 2000).

For expression analysis 2 kb upstream of bah-1 was PCR amplified with primers 5'-CTGCAGGCGAACCTTAACTGGGGAGTAC and 5'-GGATCCTGTAATCAATATATGCCTTG that added restriction sites PstI and BamHI, respectively.

All primers and added restriction sites used for these procedures are detailed in Supplementary Table S1 and Supplementary Fig. S1, A shows diagrams indicating positions and sizes of flanking sequences relative to genes of interest.

Added restriction sites for cloning into pGEX4T-2 vector are bold.

But he added restrictions so rigid that they may constitute a near-ban.

Besides, he added, restrictions like those requiring patients to obtain permission to see specialists did not save money.

California and the European Union (EU) imposed stricter limits on hydrocarbons and added restrictions on nitric oxides and carbon dioxide.

The magistrate's decision on Monday added restrictions to the bail terms, requiring that Mr. Madoff's outgoing mail be monitored by a security firm.

An associated document, partially declassified for the speech, added restrictions on drone strikes that led to a notable drop in drone attacks by late 2013.

With added restrictions on the dry weather tyres, drivers will be forced to consider tactics which prolong their longevity or face being overtaken.

Now, generally speaking, the BBC isn't allowed to encrypt or restrict its broadcasts: the licence fee payer pays for these broadcasts, and no licence fee payer woke up today wishing that the BBC had added restrictions to its programming.