Your English writing platform
Discover LudwigExact(16)
She added reaction to the duke and duchess' firstborn was likely to be unprecedented: "The bells rang across Britain when Queen Victoria had her son, the future Edward VII.
Substrate (OPD, Sigma) was added, reaction was stopped with 2 M H2SO4 and A495nm was measured on an ELISA reader (Σ960 Metertech Inc .. Phagemid DNAs recovered from six isolated clones were transformed into E. coli HB2151 cells.
To detect antibody antigen interactions tetramethylbenzidine (TMB) was added (reaction stopped at 5 min with 50 µl H3PO4 (1M)), and the resulting signal read at 450 nm (Molecular Devices EMAX micro plate reader).
We heated 10 µl samples containing 2 µg of total RNA, and 20 pmol of random hexamers (Invitrogen) at 70°C for 2 min to denature the RNA, and then chilled the samples on ice for 2 min. We added reaction buffer and M-MLV to a total volume of 20 µl containing 500 µM dNTPs, 50 mM Tris HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM dithiothreitol, 200 units of M-MLV, and 20 pmol random hexamers.
We heated 10 µl samples containing 2 µg of total RNA, and 20 pmol of random hexamers (Invitrogen) at 70°C for 10 minutes to denature the RNA, and then chilled the samples on ice for 2 min. We added reaction buffer and M-MLV enzyme to a total volume of 20 µl containing 500 µM dNTPs, 50 mM Tris HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM dithiothreitol, 200 units of M-MLV, and 20 pmol random hexamers.
This added reaction parsimoniously represents the combined effect on FX conversion of the extrinsic (FVIIa) and intrinsic (FXIa) pathway.
Similar(44)
We added reactions from alcohols to aldehydes then aldehydes to corresponding fatty acids, following [ 10].
The two added reactions represent a hypothesis that needs further experimental verification.
These differences are the direct result of added reactions and transporters.
As one increases the size of a metabolic network by adding reactions and entire pathways to minimal metabolic networks, some reactions may become non-essential because the added reactions create alternative pathways for biomass metabolite synthesis.
Examples of added reactions are EC 5.4.2.7, which is mediated by a phosphodeoxyribomutase encoded by the PGM2 gene.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com