To remove these contaminants and any residual thrombin, the C2AB tandem domains were further purified over a Superdex G75 10/300 GL gel filtration column (GE Healthcare, Uppsala, Sweden) in buffer A (150 mM KCl, 15 mM NaCl, 0.5 mM MgCl2, 20 mM HEPES, pH 7.4) with 500 mM NaCl added, and purity was determined using SDS-PAGE.
NaCl powder (99.5%% purity) was added into 0.1 mol L−1 Co(NO3 2·6H2O (99.0%% purity) aqueous solution with constant stirring for 0.5 h, ensuring achieving a weight ratio of Co/NaCl remained at 1/98.
"I'm on a State Department tour," he added, thereby ruining the purity of his witticism.
John Hays of Christie's added: "There's a purity and simplicity to Boston furniture that doesn't exist in New York or Philadelphia.
They showed all the delicious drinkability of Beaujolais, yet they were structured and balanced, with layers of complex flavors and added dimensions of depth, purity and nuance.
In order to get higher purity, we added CD2 in our purification protocol, thus CD123+CD2+CD56+ and CD123+CD2−CD56− DCs were sorted by FACS for further analysis.
Then 500 μl of 0.5 М Na2S solution (Sigma-Aldrich, USA, 98% purity) was added to the flasks which were incubated for 4 days at 28°C to produce a homogeneous clear solution of CdS nanoparticles with bright yellow color.
Stoichiometric amounts of Hf and Te powders (Alfa Aesar, >99% purity) were added to a fused silica ampoule that was typically 8 cm long with a 1.1 cm inner diameter.
SWCNT (1 2 nm in diameter, ~1 μm in length, 95%% purity), graphite oxide, and carboxylic multi-walled CNT (MWCNT, 30 50 nm in diameter, ~1 μm in length, carboxylated, 95%% purity) were added to deionized water and then sonicated for 2 h by an ultrasonic homogenizer in ice base to get fully dispersed nanocarbons in aqueous suspensions.
During the preparation of Mg-doped TiO2, 10 ml TTIP (Spectrochem, Pvt. Ltd ,India) of 98% purity was added into the 25 ml deionized water and 25 ml ethyl alcohol (Changshu Yangyuan Chemical, China) mixture with constant stirring on the magnetic stirrer.
