The phrase "added primer" is correct and usable in written English.
It can be used in contexts related to painting, construction, or any situation where a primer is applied as an additional layer or preparation step.
Example: "Before applying the final coat of paint, make sure to use an added primer for better adhesion."
Alternatives: "additional primer" or "supplemental primer".
In the two previous reports by Deng et al. and Zhang et al., cell-free vRNA synthesis could be reproduced without any exogenously added primer and host proteins, while in our cell-free system, no vRNA synthesis occurred without any added primer and host proteins, as was observed previously (Galarza et al., 1996; Seong and Brownlee, 1992; Seong et al., 1992).
3) As the authors discuss in the Discussion, un-primed vRNA-synthesis was previously demonstrated in the absence of exogenously added primer or host factors by other authors using different preparation methods of polymerase.
To determine fractions containing the IREF-2 activity at each column chromatography step, every fraction was individually assayed for RNA synthesis ability in the presence of mnRNP and the c53 model template, but in the absence of any added primer.
In this study we combined elements from the previous genotyping [ 13] and characterization [ 14] assays into a single format and added primer pairs to interrogate the gene for toxic shock syndrome toxin 1 (TSST-1) [ 19], the PVL genes (distinguishing the "R" and "H" variants) [ 20] and the region of the ileS gene that encodes low level mupirocin resistance [ 21, 22].
Add primer prior to foundation.
Add primer to the wood so that the spray paint will stick to it.
Prepare the wall as you normally would, by cleaning it off and adding primer.
I'm going to give you one look: first add primer to your entire eye, get a light green color and apply to your entire lid and make a higher cat eye, get a forest tree green color and add a small amount halfway on your lid (top/bottom doesn't matter) after add a hot pink mascara.
Controls for reactions were: (a) DNA without adding primers; and (b) primers without adding DNA (not shown) [ 29, 30].
The relational database system of ChloroMitoSSRDB 2.00 has been updated by adding primer-pair information corresponding to MISA identified SSRs, as a separate query field.
