The phrase "added prepared" is not correct and does not convey a clear meaning in written English.
It may be intended to describe something that has been both added and prepared, but the phrasing is awkward and unclear.
Example: "The chef added prepared vegetables to the stir-fry for extra flavor."
Alternatives: "included ready" or "incorporated prepped".
Afterwards 24 μl of staining solution containing DAPI (1 1000, Sigma-Aldrich) and DY-547-phalloidin (1.2 U/ml, Dyomics) was added (prepared in blocking buffer consisting of 4% BSA and 4% Sucrose in PBS).
After incubation, 90 μl of the H2O2-sample solution was mixed with 10 μl HPLC-grade methanol and 0.9 ml FOX reagent was added (prepared in advance by mixing 9 volumes of 4.4 mM BHT in HPLC-grade methanol with 1 volume of 1 mM xylenol orange and 2.56 mM ammonium ferrous sulfate in 0.25 M H2SO4).
Then the medium was aspirated, the cells were washed with phosphate buffer saline (1x PBS, 2 × 200 μL), and the pDNA complexes of TP1 to TP5 were gently added, prepared at w/w ratio of 1.6, 2.6, 3.3, 5.0, and 6.6, bPEI at w/w ratio of 1.6, and Lipofectamine, prepared following the protocol provided by the manufacturer, onto the cells.
After incubation, 90 μL of the H2O2-sample solution was mixed with 10 μL of HPLC-grade methanol and 0.9 mL of FOX reagent was added (prepared by mixing 9 volumes of 4.4 mM BHT in HPLC-grade methanol with 1 volume of 1 mM xylenol orange and 2.56 mM ammonium ferrous sulfate in 0.25 M H2SO4).
He added: "Prepare yourselves, this is serious".
He added: "I prepared for those two innings that I threw like I prepare for a regular-season game.
"It's the ABC," I added, not prepared to push any more.
"The government," he added, "is prepared to demonstrate that there was no immunity agreement barring the prosecution of Mr. Rosario".
He added: "We prepared well.
To test the first scenario, we added freshly prepared hematin-saturated wet octanol to the top solution layer.
