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Briefly, denatured but non-reduced culture fluid samples were resolved in 7.5% SDS PAGE gels prepared with the added incorporation of gelatin (1 mg ml−1) or β-casein (1 mg ml−1) prior to casting.
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During the last 18 hours 3H-thymidine was added and incorporation was measured and expressed as mean stimulation index (CPM Antigen/CPM background)±SEM.
In one experiment, phagosomes were incubated with only unlabelled ATP and after different times of pre-incubation, γ32P.ATP was added and incorporation of P into PIP was analyzed (Fig. 4B, left panel).
An equal volume of 10% TCA was added to terminate incorporation.
The senescence status was verified by staining for senescence-associated β-galactosidase (SA-β-gal) activity, by the appearance of a characteristically enlarged and flattened morphology (Fig. S1, Supporting information), and by the lack of BrdU incorporation, added to the culture medium for 48 h (Fig. 1D and Fig. S1, Supporting information).
Notably, the anti-androgen bicalutamide (Bic) increases BrdU incorporation, whereas it very slightly affects BrdU incorporation when added alone to the cell medium.
This paper describes recent advances in sensor design, characterization, and detection of biomolecules for applications in bioterrorism surveillance and medical diagnostics, with an added emphasis on the incorporation of emerging capture agents that give improved sensor performance.
Also, scale adhesion in cyclic testing was improved with the co-addition of Hf with La or Y. Ti was added to investigate its incorporation in coatings on superalloys containing significant Ti additions.
BK or Iso were added to the [P] incorporation buffer to test their effect on the phosphorylation of cTnI.
The manuscript would be improved if some evidence such as caspase staining or BrdU incorporation was added.
After 3 days of incubation, 1 μCi/well [H]thymidine was added overnight and thymidine incorporation was measured using a β-scintillation counter.
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