Sentence examples for added in triplicate to from inspiring English sources

Following this incubation, the mixture was added in triplicate to a 96-well black-sided plate (100 μL/well).

The mixture of insulin and the anti-insulin antibody was added in triplicate to the insulin-coated wells, and the plate was incubated for 1 h.

100 μl samples of each CD4 T cell culture were added in triplicate to a 96-well plate and incubated for 6 h with 1 μCi per well of [3H] thymidine.

200µL plasma was diluted with 400µl binding buffer and added in triplicate to the 96-well plate.

Briefly, 100 µL of bacterial suspensions (100 bacteria/cell) were added in triplicate to the wells coated with HT29 cells.

Using the same tissue extract as described above, 20 µl aliquots were added in triplicate to wells of a 96 well UV transparent microtitre plate (Costar).

0.1×106 Treg of Group 1, Group 4 were then added in triplicates to the cultures.

The medium was then removed, and 100 µl of the peptides investigated (at 60 µM, diluted in SFM/BPE-rEGF or in keratinocyte-SFM supplemented with 20% human serum) added in triplicates to different wells of the plate.

For analyses of the capacity of serum to inhibit binding we used 2×105 tritium-labeled late-stage IE and 15 µl serum in a total volume of 120 µl which were added in triplicates to wells coated with 2 µg/ml of the commercially available chondroitin sulfate proteoglycan Decorin (D8428; Sigma-Aldrich).

Selective NMDAR antagonists, MK-801, and memantine were then added in triplicates to each well at varying concentrations, followed 5 minutes later by the administration of the NMDAR agonists, QUIN and glutamate at excitotoxic levels of 500 nM and 500 µM respectively.

Broncho-alveolar lavage (BAL) fluids in PBS pH 7.4 were added in triplicates to antibody coated plates for IL-5 detection.