Sentence examples for added in duplicate to from inspiring English sources

The supernatants were added in duplicate to appropriate pre-coated plates.

Diluted supernatants were added in duplicate to confluent monolayer cultures of Vero E6 cells and incubated for 1 hour at 37°C with periodic rocking.

For poliovirus titration, 10-fold serial dilutions (1∶10 to 1∶109) of the culture medium were added in duplicate to HeLa cells cultured in 48-well plates.

Aliquots of 6.7 µl were taken from a homogenate dilution series and added in duplicate to the wells of a microtiter plate.

Samples, a positive (a pool of hyperimmune serum collected from a malaria endemic area) and negative controls (serum from European malaria naïve volunteers) were added in duplicate to each plate.

After blocking with 1% BSA, plates were washed with PBS-T (PBS with.05% Tween20) and 100 µl of 1∶100 diluted samples were added in duplicate to each ganglioside and control wells.

BRO was added in duplicates to reach final concentrations of 1, 3, 10, 30, 100 and 300 μg∙mL− 1 5 min prior to addition of A23187 at 15 μg∙mL− 1.

The compounds were diluted in DMSO to 12 concentrations and 18 µl of each dilution was added in duplicates to a Corning 3706 plate.

Cells were added in duplicates to the wells in 3-fold serial dilutions, starting at 2,000 cells/well for hybridoma cells, 1×106 cells/well for splenocytes and 1.3×106 originally cultured cells/well for the in vitro stimulated cells.

50 µl of TNF-α standard [1 ng/ml] (CHEMICON) as a reference and 15 µl of serum samples were added in duplicates to the cells and serial dilutions were performed.

40 µL of whole blood was diluted 1∶5 in RPMI 1640 L-glutamine (2 mM), penicillin (100 IU/mL) and streptomycin (10 mg/mL) (Gibco, Invitrogen) and added in duplicates to 96-well plates supplemented with Ag85A, Ag85B, TB10.4 antigens, PPD or BCG as described above.