"added in a volume of" is correct and usable in written English.
You can use it to refer to something that has been included in a book or other written publication. For example: "The author included an additional chapter added in a volume of the book's third edition".
Standard, unknown samples and no template control (NTC) were added in a volume of 5 µl.
For binding inhibition assays, the indicated concentrations of telomestatin were added in a volume of 1 µL.
Cells were added in a volume of 50 µl onto the glasses, incubated for 5min at 37°C and fixed by direct addition of PFA.
For measurement of cyclic nucleotides, the pan-phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX) was then added in a volume of 10 µl to give a final concentration in the well of 300 µM for 15 minutes.
Stimulants were added in a volume of 100 µl (1 µg/ml final concentration for each of 21 ESAT-6 peptides or 10 µl/ml final concentration of tuberculin (PPD, SSI, Copenhagen, Denmark)).
Denatured, whole RNA (2 μg) was added in a volume of 3 μl.
Test specimens were produced after adding in a volume of 1.5%% of the total mix volume.
Briefly, lysis buffer (8 M urea, 75 mM NaCl, 50 mM Tris, one tablet of Complete Mini protease inhibitors cocktail [Roche Diagnostics, Bromma, Sweden] and 10 mM sodium pyrophosphate) was added in a volume ratio of ∼3:1 buffer to cell pellet.
Washed and sterilized zirconium oxide beads (0.5 mm diameter, Next Advance, Cambridge, MA) were added in a volume equal to that of the tissue.
We optimized the liquid medium in terms of concentration of sucrose, amounts of 2,4-D, pH, and types of nitrogen nutrients added in a total volume of 50 mL of MS medium.
1.5 μg of pure recombinant VDR (or Runx2) proteins were added in a final volume of 500 μL of the binding buffer containing 0.5% of non-fat milk, and were subsequently incubated for 2 h at 4°C with gentle agitation.
