Sentence examples for added for concentration from inspiring English sources

A series of peptides was synthesized on the basis of model Baldwin α-helical peptides, Ac- YG AKAAAAKAAAAKAA(GY)-NH2, with the Tyr Ac- YG AKAAAAKAAAAKAAon determination and located at the N-terminus (for peptides with C-terminal modifications) or C-terminus (all other peptides) as appropriate to avoid interactions with the sites of modification.

Heparin (average MW = 4400, Celsus, EN-3225) or fluorescently conjugated heparin (average MW = 18 000, Invitrogen, H7482) was then added for final concentrations of 50 and 2.5 μM, respectively.

Then, 4 h later, lipopeptides were added for a final concentration of 65.76 µM, and the plates were maintained at 37 °C for 48 h.

DSS in DMSO was added for a final concentration of 2 mM.

To this, R-α-OH-C16-ceramide from MUSC Lipidomics Core was added for a final concentration of 0.3 mM.

10 µl of Gly-pro-7-amido-4-methylcoumarin hydrobromide (Gly-pro-AMC) was added for a final concentration of 200 µM (Sigma, St . Louis MO).

After the total amount of extracted protein was measured by a micro BCA (bicinchoninic acid) protein assay (PIERCE, Rockford, IL), DTT was added for a final concentration of 10 mM.

After 2 hours of incubation at 37°C, an equal amount of relevant complete medium was added for a final concentration of Fe to pro, 50 µg/ml to 3 µg/mL, respectively.

After 2 hours of incubation at 37°C, an equal amount of complete medium was added for a final concentration of Fe to Pro, 50 µg/ml to 3 µg/mL, respectively.

Overnight cultures were diluted 1∶100 into 200 µl of fresh Luria Broth (LB) in 96-well microplates and 10 µl of paraquat stock solution was added for a final concentration of 250 µM.

The pellet was air dried and dissolved in 1 ml CaCl2, then 1 ml 40 mM Tris pH 8 with 2% Triton-X 100, was added for the final concentration of 20 mM Tris and 1% Triton-X.