Sentence examples for added during the initial from inspiring English sources

On the other hand, the use of auto-induction media whereby lactose added during the initial media preparation acts to automatically induce protein production (Studier 2005) and thereby circumvent the need for monitoring cell growth and addition of inducer, should allow for a more automated and efficient production method.

Fetal calf serum (FCS; 5%) was added during the initial 24 hour attachment phase and then serum-containing medium was removed and replaced with medium containing 20 ng/ml epidermal growth factor (Invitrogen).

For C2-ceramide treatment, 50 µM C2-ceramide was added during the initial 2 h serum starvation prior to the pulse-labeling of cell surface GLUT4 myc and remained present during the 30 min re-warm.

For experiments with phytochrome, 10 μM PCB was added during the initial dilution to 0.2, as this concentration was determined sufficient for maximal activation with a 4 h incubation time.

Because of the rarity of the manifestation (late reactivation) and location (gallbladder), plus a residual abscess shown on CT scan 8 weeks after surgery, prolonged treatment with doxycycline and rifampin was administered for 3 months, with gentamicin added during the initial 2 weeks (4 ).

Initial codes were developed to match the interview topics, and additional codes were added during the review and coding process as needed.

Several radars were added during the war.

Exterior spotlights were added during the 1980s.

No nutrients were added during the experiment.

No chemicals were added during the pretreatment.

*No enzyme added during the model SSCF.