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To a solution of 11a (400 mg, 550 μmol) in EtOAc (15 mL) at 0 °C was added concentrated HCl (5.4 mL).
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An NLS was also added, concentrating the protein in the nucleus, which simplifies analysis and increases the signal-to-noise ratio.
The volume of the buffer and the cells was taken into account, and the salt concentration was increased to 400 mM by gently adding concentrated 5 M NaCl dropwise while mixing.
That's when American manufacturers started adding concentrated milk solids, a cheap byproduct of butter and cheese production, and when advances in refrigeration made it possible to freeze the mix quickly to lower temperatures with vigorous agitation.
The restoration of surface motility of the rhizobactin-defective mutants was attempted by adding concentrated supernatants containing rhizobactin 1021.
You can also add concentrates after sticking in a little aluminum disk into the heating chamber.
As this in vitro study should represent normal feeding conditions it was necessary to add concentrate provided by DEUKA Schaffutter, Erfurt, Germany.
He added: "I concentrated harder than I possibly would if I was in good touch".
The stain solutions were prepared after the following procedure: Solution A, consisting of 0.01 g azure A and 0.05 g cresyl violet acetate dissolved in 50 ml distilled water, was mixed with solution B, based on 0.05 M Tris in 45 ml distilled water, to which was added dropwise concentrated hydrochloric acid until pH 7.5 was achieved.
One tenth volume of 2 M L-proline was added before concentrating the protein using 30 kDa cutoff Amicon Ultra concentrators.
"We're definitely trying to tap into the same emotional insight," he added, by concentrating the campaign in the social media favored by the target audience.
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