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Spiking of control cattle tissue with different numbers of BCG cells showed that recovery from these measured by real-time PCR was as low as 22% of the theoretical added colony forming units (cfu), determined by culture.
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The H0 expectation was derived from the naïve foragers that were added as colonies were hived as swarms at location B (see above).
Prior to fermentation, pre-culture cells were grown by adding one colony from the plate to liquid media containing 10 g/L each of glucose, yeast extract and peptone.
Incipient social insect colonies undergo a period of ergonomic growth in which new workers are added to the colony but no sexuals are produced [ 34].
The Aro Confederacy ended and the rest of Aro dominated areas was added to The Colony and Protectorate of Southern Nigeria, declared in 1900.
The pH was adjusted to 5.0, and 0.1% Triton X-100 (Merck) was added as a colony growth limiter.
Wild moths (approx 10 per year) were added to the colony each summer in order to ensure outbreeding.
Unmarked, newly emerged, wild-type bees were added to each colony for 3 days following the introduction of the marked, focal bees.
This colony is of mixed ancestry from several invasive populations of A. glabripennis obtained within the U.S. and has been in culture for over 10 years, but field-collected insects from regulated areas in the U.S. are routinely added to the colony to maintain genetic diversity.
The proposed solution scales very easily and so adding more Colonies could sufficiently seed up the processing.
Inoculations were carried out by adding 50 colony-forming units (CFU) of M. cannonballus per gram of sterile soil as described by Iglesias et al. [ 94].
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