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Anhydrotetracycline (Sigma-Aldrich, Germany) was added as appropriate.
VB6, L-Trp and arabinose were added as appropriate.
Ampicillin (Meiji Seika Pharma, Tokyo, Japan, 100 μg/mL) and kanamycin (Nacalai Tesque, Kyoto, Japan, 25 μg/mL) were added as appropriate.
Alkaline amendments are added as appropriate in the upper portions of graded CCPW before putting the soil cover and vegetating it (Daniels et al. 1999).
Cells were routinely grown aerobically in Luria-Bertani (LB) broth at 37°C, and antibiotics were added as appropriate (ampicillin at 100 μg/ml, chloramphenicol at 10 μg/ml, erythromycin at 1 or 100 μg/ml, and neomycin at 10 μg/ml).
5-fluoroorotic acid (5FOA) was added as appropriate at 1 g/liter (Zymo) or geneticin (G418) was added at 200 µg/ml.
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Second, TB health professionals working in TB dispensaries were asked to comment on the draft instrument, and to add as appropriate, other indicators and operational definitions.
Trace elements, minerals and vitamins were added as clinically appropriate.
The QIs were reviewed once a year, at which time existing QIs were modified, or discontinued if they lacked discriminatory power, and new indicators or subindicators were added, as deemed appropriate by the scientific advisory board.
To quantify IAA and ACC, [C6]-IAA and [H4]-ACC, respectively, were added as internal standards in appropriate amounts before extraction.
Once the set of basic building blocks is implemented, interactions between biological elements of the model can be changed by replacing one building block by another, and new elements can be added as instances of the appropriate kind of building blocks, all this with minimal changes in the implementation of the model.
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CEO of Professional Science Editing for Scientists @ prosciediting.com