Sentence examples for added and subsequent from inspiring English sources

This efficient synthesis involves the growth of lithium titanate hydrate (LTH) precursors on the graphene oxide (GO) by a hydrothermal reaction, assembly into LTH-GO film by vacuum filtration with some extra GO added, and subsequent conversion into LTO-rGO nanocomposite film through calcination.

After incubating for 30 minutes, HRP substrate was added and subsequent steps were as for the NN ELISA.

After 1 min of mitochondria preincubation, 0.7 μM BTM-P1 was added and subsequent oxidation of endogenous NAD(P H was observed.

The anti-SSEA-1 ascites fluid diluted 1 1,000 in PBS was added and subsequent steps were carried out using DAKO universal LSAB® kit, Peroxidase (DAKO, USA) according to the manufacturer's instruction.

After washing with 1 × TBST three times for 10 min, secondary antibodies were added and the subsequent protocol was identical to the western blotting (paragraph above).

After washes in PBS + 0.1% Tween-20, Goat anti-Rabbit Immunoglobulins-alkaline phosphatase (Southern Biotech, Birmingham, AL) was added and after subsequent washes the blots were developed using 5-bromo-4-chloro-3-indole-phosphate/nitro blue tetrALolium (Southern Biotech, BirminghAL, AL).

But Redding and Shaw said the added ejection and subsequent suspension had had a desirable effect.

At this stage, both 1 mM EDTA and 5 mM DDT was added and kept in all subsequent buffers.

Mr. Cameron added: "That evidence and subsequent interviews with the personnel involved" — believed to have included a Navy Seals unit specializing in hostage rescues that has participated in numerous special forces raids in Afghanistan — "suggest that Linda could have died as a result of a grenade detonated by the task force during the assault.

Thirty-two unique oligosaccharides may be added to Asn297, and subsequent random pairing of heavy chains could generate almost 500 glycoforms (Jefferis, 2009).

All co-authors commented on and added to this and subsequent drafts to produce a final manuscript.