Sentence examples for added and mixtures from inspiring English sources

Glass beads were added, and mixtures were sonicated for 10 pulses of 30 s each at maximum intensity using a Ultrasonic Processor sonicator equipped with a microtip.

After 48 h of incubation, 20 μL of MTT reagent (5 mg/mL) were added and mixtures were reincubated for 4 h.

Mixtures were heated to 80°C, 1U of thermostable Taq DNA polymerase (Pharmacia) was added, and mixtures were subjected to 35 cycles at 95°C for 30 s, 55°C for 45 s, and 72°C for 240 s.

Next, 0.5 μL Tris (0.5 M, pH 8.3) and 0.25 μL alkaline phosphatase [Sigma; 50 units/mL in 2.5 M (NH4 2SO4] were added and mixtures were incubated for an additional 2 h at 37°C.

About 20 μg yeast tRNA (Invitrogen, Carlsbad, CA) and 20 μg polyadenylic acid (Sigma Aldrich, St . Louis MO) were added and mixtures were heated at 100°C for 1 min, placed on ice for 2 min and at 42°C for 5 min.

H2O was added and mixture was extracted with EtOAc.

DNA was added and reaction mixtures were incubated 15 min at 37°C; heparin was added and reaction mixtures were incubated 2 min at 37°C; and NTPs were added and reactions mixtures were incubated 10 min at 37°C.

Sugar is added and the mixture is filtered.

Four mls CHCl3 were added and the mixture shaken.

3 M sodium acetate was added, and the mixture vortexed.

One volume of CHCl3 was added, and the mixture vortexed.