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Schöniger flask combustion involves a special Erlenmeyer flask to which is added a small volume of a suitable solution (often a dilute sodium carbonate or sodium hydroxide solution) that absorbs and retains the inorganic analytes.
However, in previously conducted experiments I did not observe unacceptable froth and turbidity when I added a small volume of E. coli cells beneath liquid following the 2011 VCC method, and I had already proven that the 2005 VCC method resulted in cross-contamination due to aerosol formation based on experiments I conducted in 2013.
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We have shown that adding a small volume change to classical rubber elastic models can aid in analytical and numerical simulations of polymer structures.
The level II composite was obtained by adding a small volume fraction (vf) of the ball-milled level I composite to Mg using the powder metallurgy route followed by microwave-assisted rapid sintering and hot extrusion.
Chemical reduction, in order to generate the reduced Fe(II)/Fe(III) in the FeS clusters, was performed by adding a small volume of anaerobic sodium dithionite solution to the protein solution under a weak stream of argon gas (20-fold excess dithionite with respect to the protein).
Osmotic stress (0.4 M NaCl) was produced by adding a small volume of concentrated NaCl solution to the yeast culture 10 min before cross-linking with formaldehyde.
To examine the average size of these particles, a bright-field microscopy technique was performed using a Leica DMLM microscope by adding a small volume of spheres on the surface of a microscope slide.
The ferrous form was obtained by adding a small volume (2 5 μL) of a fresh sodium dithionite solution (10 20 g/L Na2S2O4·2H2O) to 40 μL of a deoxygenated protein solution.
In contrast to aqueous procedures, we do not adjust the first point to a specific pH and only rarely will initially add a small volume of the opposite titrant.
After 4 hours, wash the cells twice with PBS and add a small volume (3 ml) of culture medium to avoid excessive dilution of autocrine growth factors produced by the RCE cells.
Co II Cbl and Co II Cbi+ were prepared by adding a small volume of saturated HCOOK to degassed solutions of H2OCbl+ and (H2O 2Cbi2+, respectively, and the progress of the reduction was monitored spectrophotometrically.
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