As this in vitro study should represent normal feeding conditions it was necessary to add concentrate provided by DEUKA Schaffutter, Erfurt, Germany.
The volume of the buffer and the cells was taken into account, and the salt concentration was increased to 400 mM by gently adding concentrated 5 M NaCl dropwise while mixing.
That's when American manufacturers started adding concentrated milk solids, a cheap byproduct of butter and cheese production, and when advances in refrigeration made it possible to freeze the mix quickly to lower temperatures with vigorous agitation.
The restoration of surface motility of the rhizobactin-defective mutants was attempted by adding concentrated supernatants containing rhizobactin 1021.
An NLS was also added, concentrating the protein in the nucleus, which simplifies analysis and increases the signal-to-noise ratio.
Add Glögg concentrate, orange peel, raisins and spices as in the previous method.
Vitamins (1∶50) and amino acids and Glutamax (1∶100) were added from concentrates (Invitrogen, Carlsbad, CA).
Sodium pyruvate (2 m m), MEM amino acids solution (50×, without L-glutamine) and MEM vitamins solution (100×) were added from concentrates (Fisher Scientific, UK).
Sodium pyruvate (2 mM), MEM amino acids solution (50X, without L-Glutamine), and MEM vitamins solution (100X) were added from concentrates (Fisher Scientific, UK).
One tenth volume of 2 M L-proline was added before concentrating the protein using 30 kDa cutoff Amicon Ultra concentrators.
