Sentence examples for add and Rotate from inspiring English sources

Add and Rotate Oils.

Antibody was added and rotated overnight at 4 °C.

In the next step (b), the benzene ring is added and rotated in a perpendicular orientation with respect to the initial fragment.

Next, 50 µl of protein A/protein G-Plus agarose beads were added and rotated for 90 min at 4°C.

After magnetic capture of the beads, the supernatant containing blocked phage was combined with the toxin mix and rotissaried for 1 h. 10 µL of beads that had been blocked in 1 mL of PBSBT were magnetically captured, the supernatant removed, the phage/toxin mix added and rotated for 30 min to capture the biotinylated toxin and any specifically bound phage.

Treg were cultured for 24 h with CD3/CD28 beads + hrIL-2 and were labeled with 5 µM CFSE. 100 µl of Treg (105) were added and rotated over the sections for 45 min. Unbound cells were removed by washing in PBS.

Then, protein A/G PLUS agarose beads (Santa Cruz Biotechnology) were added and rotated for 1 h.

Protein A sepharose beads (50  μl) were then added and rotated for 3 h at 4°C.

Protein G Sepharose beads (Protein G Sepharose 4 Fast Flow, GE Healthcare) were added and rotated at 4°C for 1.5 h.

Protein A-sepharose beads were added and rotated at 4°C for 1 h, followed by four to five washes in lysis buffer.

Protein A beads bound to 50 µg human Fc control protein or 50 µg IL1RAPL1 ecto-Fc protein were added and rotated overnight at 4°C.