Finally, we also find that the wrapping process can be completely retarded by adding a substrate below the graphene flake.
The batch test gives values for biogas production in an ideal case, without adding a substrate to the original during the process.
To obtain basal OCR, we first added a substrate to the preparation that contained pyruvate (10 mM) and malate (2 mM).
After washing of unbound ACE, plate-bound ACE activity was measured by adding a substrate for ACE (Hip-His-Leu) directly into wells [33], [36].
A color reaction was developed by adding a substrate solution containing 3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidine.
After adding a substrate, the reaction was evaluated as OD450 values.
After 3 washes, color development was initiated by adding a substrate solution and was monitored at 450 nm using a microplate reader (Emax, Molecular Devices).
This specific compound or protein can then be quantified by adding a substrate for the reporter enzyme and measuring the concentration of the product [ 22].
The labelled secondary antibody was visualised by adding a substrate containing diaminobenzadine (Zymed), and sections were counter-stained, dehydrated and mounted.
Add a background, substrate and decorations.
The previously described experimental design in Figure 3 is a system in which we add a peptide as a substrate for the truncated enzyme to measure the rate of thiol/disulfide exchange between the two enzyme redox centers.
