Sentence examples for adapter table from inspiring English sources

The 3' RACE cDNA synthesis reaction was primed with the 3' RACE Adapter (Table S1).

An attB1 adapter (Table S1) was ligated to the 5-prime end of the double-stranded cDNA, and the Qiaquick PCR purification system (Qiagen, Valencia, CA) removed residual un-ligated adapters and small (<100 bp) cDNA molecules.

For the first round of amplification of the 3' end, the cDNA reaction primed with the 3'RACE Adapter (Table S1) was used as template and 3'RACE Outer Primer and AmNrx1ex28_5'_2 were the primers (Table S1).

Then, PCR duplicates were collapsed using the six Ns present on each adapter (Table 1).

Initially, the humidification rate of the additional humidification system was set at 20 ml/h saline solution via the jet adapter (Table 3).

Reverse transcription (RT) of polyadenylated miRNAs was performed as previously described [47] using universal stem-loop RT adapter (Table S4) and the resulting product was serially diluted and stored at −20 °C until use.

The resulting DNA population reproducibly exhibits a relatively narrow size distribution (Figure 2), and can be used to prepare high-throughput sequencing libraries by ligating appropriate oligonucleotide adapters (Table 1).

Adapters (Table  2) were changed between cross-hybridization, and the different products were purified using the GFX kit.

Thereafter, Illumina indexing adapters (Table  6) were ligated according to the standard protocol for pooling of samples prior to sequencing and for subsequent identification of pooled samples in downstream analysis.

Isolated RNA from feline bone marrow was reverse transcribed into cDNA according to manufacturers protocol (Superscript II, Invitrogen) using oligo d(T) linked to an adapter sequence (Table 1: 3'-RACE Adapter Primer).

We obtained 61,572,264 and 39,250,410 total reads from T. pseudonana and F. cylindrus, respectively after removing adapter sequences (Table  1).