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Reads with adapter contamination, potential contaminant, and poor-quality reads with ambiguous sequences "N" were discarded.
Discard reads with adapter contamination.
Reads with adapter contamination were trimmed.
Firstly, adapter contamination in the raw data was removed.
Any reads that had adapter contamination in the middle were discarded as possible chimeric sequences.
Only reads with adapter contamination were filtered out using FilteR [ 24].
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The resulting directional 100 bp paired-end reads were quality-checked with FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/), and adapter contaminations and low quality tags in the raw data were removed.
FilteR was developed using C++ to detect adapter sequence contamination as well as poor read quality.
Raw sequence files were trimmed to remove adapter sequence contamination and low-quality sequences (Trimmomatic [ 44], Fastx toolkit).
In the case of the cobalt-chromium alloy neck adapter, the contamination of the joining area did not influence the micromotions significantly (p = 0.43).
Thereby, the adapter dimer contamination, the sample loading efficiency, the obtained average read-length and the number of filtered sub-reads have been assessed.
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