Sentence examples for adapter by from inspiring English sources

For each sample, the 18-30nt small RNAs were ligated with 5'- and 3'- RNA adapter by T4 RNA ligase after being purified by polyacrylamide gel electrophoresis.

Restriction fragments were subsequently blunt-ligated with 50 pmol of double strand adapter by incubating 10 μl of the genomic digest with 10 U of T4 DNA ligase and 2 μl of its buffer in a final volume of 20 μl overnight at 16°C.

For each sample, the 18-30 nt small RNAs were ligated with 5' and 3' RNA adapter by T4 RNA ligase (TaKaRa) after they were purified by electrophoretic separation on a 15% TBE-urea denaturing PAGE gel, and at each step purified by urea PAGE gel electrophoretic separation.

This strategy is more powerful than alignment -like) alignment -like for identifying approachesarts in single reads, which frequsedly remove sequence form the read ends that match the adapter by chance, or whidentifyingidentify readapterter startsce due to the hinher singlecing ereadsat the end of reads.

Remove the barrel and barrel adapter by wiggling and pulling on it.

Power on your phone adapter by plugging in the power cord to the back of the adapter and the plug into the wall outlet.

Users can identify the faulty power adapters by looking underneath the part that is inserted into the power socket, which has either four or five characters or no characters printed inside it.

The T4 RNA ligase 2 (Rnl2) shows optimal activity on double-strand nucleic acids [14], though it is also used for adding a 3'-adapter by single-strand ligation in small-RNA cloning with the Rnl2(1 249) truncated enzyme producing reduced ligation side products [15], [16].

The sheared DNA was end-repaired and quantitated before attachment of the the SOLiD P1 and P2 adapters by ligation.

In brief, DNA samples were fragmented and tagged with adapters by Nextera XT transposase.

The multiple complementarity of the proto-adapters (by considering U-G pairs) increases the synonymy of the reactions.