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We adapted the alignment and classification methods to data from next-generation sequencing techniques to provide reliable information.
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This challenge has been extensively addressed over the last years and several algorithms are available that either align the reads in a 3-letter space or adapt the alignment scoring matrix in order to account for the C/T conversions.
By adapting the profile alignment algorithm, the alignments from the STAP database remain intact, while gaps are inserted and nucleotides are trimmed for the query sequence according to the profile defined by the previous alignments from the databases.
The fitness function used adapted the Needleman-Wunsch alignment algorithm (a dynamic programming algorithm used to efficiently find similar regions between two sequences of DNA, RNA or protein. It can be used to efficiently compare code fragments) to increase the similarity between the two sets of code, allowing the derived refactoring sequences to remove code smells.
We have adapted the multi-genome alignment tool EvoPrinter to facilitate discovery of HGT DNA sequences within bacterial genomes and within their mobile genetic elements.
Contrary to LANE runner v1 (Tzika et al. 2011) that used MUSCLE (Edgar 2004), LANE runner 2.0 uses Clustal Omega which is better adapted to the alignment of small fragments against a long reference sequence.
After establishing the accuracy of the single exome analysis, we focused on adapting the Novoalign alignment pipeline for integration with SPLINTER.
By recasting the problem of redundancy in multiple structural alignments into this framework, in which the matrix A now describes the multiple alignment, we adapted the QR factorization to produce a minimal basis set of protein structures which best spans the evolutionary (phase) space.
We adapted this alignment strategy to our segment-based approach using an existing graph-theoretical optimization algorithm and combined it with our previous 'direct' greedy approach.
The adapted alignment method with the suitable parameters resulted in reliable models.
Maximum likelihood analyses were performed using RAxML 7.0.3 [ 98] adapting the program parameters to the alignment as described in the manual ("hard & slow way" - with 10 parsimony starting trees and 6 different rate categories).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com