Morgan Freeman and Anthony Edwards will be among the actors performing in "8," written by Mr. Black, the Academy Award-winning screenwriter of "Milk," and adapted from reporting and trial transcripts from the case of Perry v. Schwarzenegger, in which a federal judge in San Francisco struck down California's Proposition 8 in 2010.
We produced NHU cell derivatives that were transduced with a retrovirus expressing a β-catenin-specific short-hairpin RNA (shRNA) adapted from a reported siRNA sequence [25].
An enzymatic decellularization protocol, adapted from previously reported methods [34], was applied.
The stimuli and procedure for the gap task were adapted from those reported in our previous study (16).
Participants fasted for 10 to 12 hours and performed a simple task adapted from one reported previously (34,42).
Adapted from previously reported instruments (2, 9, 10), the L-PAQ captured detailed information on 3 primary domains of physical activity and specifically utilized an extensive, open-ended list of sports/recreational activities, plus an open-ended format for occupation that permitted respondents to report the specific activities in which they regularly engaged.
On day 2, the medium was changed to a composition adapted from work reported previously (Kennedy et al., 2007): Stempro34 (Invitrogen, 10640019) containing 200 ng/ml VEGF (Peprotech, 100-20), 5 ng/ml bFGF (Peprotech, 100-18B), 10 ng/ml BMP4 (R&D Systems, 314-BP-050), 0.4 mM monothioglycerol, 50 µg/ml ascorbic acid, 2 mM L-glutamine (Invitrogen, 25030-081) and Pen-Strep (Invitrogen, 15140-163).
This method was adapted from a literature reported method [18].
The MQN-space approach was used to classify and represent the fragrance-like chemical space in form of an interactive application, the FL-mapplet, which is adapted from a previously reported MQN-mapplet application for the focused FL chemical space (freely available from http://www.gdb.unibe.ch) [40, 41].
This assay was adapted from the method reported by Karmali et al. [23].
