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For Experiments 4 and 5, we adapted a protocol for discrimination learning for adult fish: two stimuli were repeatedly introduced at opposite ends of the home tank, one reinforced with food.
To identify novel proteins and develop antibody probes for detection of subcellular compartments in Neospora caninum, we adapted a protocol for purifying a mixed fraction of organelles from T. gondii and isolated a similar fraction from N. caninim (Figure 1) [24].
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To this end, we adapted a protocol developed for cDNA library normalization [26], in which highly abundant sequences are degraded by a duplex-specific nuclease based on their re-annealing kinetics.
To carry out ChIP-sequencing with T. reesei, we adapted a protocol developed for Neurospora crassa (Tamaru et al. 2003; Smith et al. 2011).
We therefore adapted a protocol used to manually screen for precocious 2° islet formation at 5 dpf (Rovira et al., 2011) to the task of detecting increased β-cell numbers (>YFP fluorescence) via ARQiv.
We adapted a protocol to prepare isotopically uniform steroids for use as a calibrant in GCC-IRMS that can be analyzed under the same conditions as used for steroids extracted from urine.
Methods were adapted from a protocol for modifying visible-light dissecting stereomicroscopes for GFP (Ian D. Chin-Sang, personal communication; ).
This study aimed to implement, evaluate and adapt a published protocol for the screening and management of IPV and to recommend a model of care that could be taken to scale in our underdeveloped South African primary health care system.
To map the adduction sites of Pin1 by HNE, we adapted a previously reported protocol for determining the adduction sites of cytochrome c.
Fractionation was adapted from a protocol written for cultured cells obtained from the lab website of David Ron (http://ron.medschl.cam.ac.uk/protocols/NucCyto.html).ac.uk/protocols/NucCyto.html
MRE with an adapted protocol for pregnancy is a reliable imaging modality to manage in pregnant women with known or suspected CD.
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