However, only six parameters (intracellular spontaneous firing rate, Sag, Fmax, AHPs amplitude, AP adaptation ratio, and APex duration) were significant for all three pairs of groups tested.
Statistical analysis of these groups revealed that six of the parameters (intracellular spontaneous firing rate, Sag, Fmax, AHPs amplitude, AP adaptation ratio, and APex) significantly divided the population into three groups.
Ratios between the first and the tenth ISI were defined as adaptation ratio 1 and between the first and the last ISI as adaptation ratio 2. Coefficients of variation (CV) were examined for the peak amplitude, HD, ISI, maximal rate of rise, and rate of decay of action potentials during a train.
Furthermore, seven physiological parameters (action potential [AP] half-width, firing frequency per 100 pA, membrane time constant [τM], inter-spike interval [ISI], adaptation ratio, AP, and after hyperpolarization [AHP] amplitudes) were added to the CA. This facilitated a clear separation of the interneuron control group from the excitatory neurons.
The cold adaptation ratio (total cold adapted proteins/total hot adapted proteins) is shown to be significant above a threshold of 1.0 (right).
The responses of GP neurons to varying levels of current injection via a whole-cell pipette were used to extract the size of the sag of the membrane potential, the rebound firing, the mono- or biphasic AHP, the pattern of firing in response to injected depolarizing currents, and the AP adaptation ratio.
Each of the 2038 proteins was classified as significantly cold or hot adapted for a given parameter using χ2 tests for the sum of cold adapted proteins vs total hot adapted proteins and was used to define the cold adaptation ratio for P. halocryophilus Or1 genes.
Assessing genome-wide cold adaptation has effectively been used to describe global adaptive features of cryophilic proteins and can be defined by the cold adaptation ratio that distinguish proteins with greater flexibility or thermal stability at colder temperatures based on the amino acid content (Ayala del Rio, 2010).
Secondly, the adaptation ratio models of two mutations are fully analyzed and compared.
The adaptation ratio was calculated as the ratio between the first and the last interspike interval during a depolarizing current step 60 pA above the rheobase.
The action potential adaptation ratio was calculated as the ratio between the last and first action potential amplitudes in depolarizing current steps of 50 pA.
