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We used genome-wide transcriptional profiling to dissect the initial events accompanying the adaptation of glucose grown H. polymorpha cells to methanol metabolism.
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18 19 Serum glucose level was determined with a standard adaptation of the glucose oxidase peroxidase chromogen-coupled system foxidase peroxidase chromogen-coupledal fluidsystem
Serum glucose level was determined with an adaptation of the glucose oxidase-peroxidase-chromogen-coupled system for glucose determination in biological fluids.
Fasting glucose was measured at each examination by rate reflectance spectrophotometry using thin-film adaptation of the glucose oxidase method on the Vitros analyzer (Johnson & Johnson Clinical Diagnostics, Rochester, NY).
Fasting serum glucose was measured at each exam by rate reflectance spectrophotometry using thin film adaptation of the glucose oxidase method on the Vitros analyzer (Johnson & Johnson Clinical Diagnostics, Rochester, NY).
Future studies are required to investigate dynamic adaptation of blood glucose targets over time.
This observation is consistent with previous studies (19, 30) and supports the notion that there is no adaptation of the glucose level at which cognitive dysfunction occurs (30).
After 48 h in low glucose concentrations, the added insult of PDT induces cell death, demonstrating that the protective effect of chronic adaptation to glucose deprivation is overwhelmed.
Plasma glucose concentration was measured using an adaptation of the hexokinase-glucose-6-phosphate dehydrogenase method (Dade Behring, Milton Keynes, UK).
Kidney adaptation was characterized by a lower rate of glucose reabsorption, whereas pancreatic adaptation was associated with a larger number of small islets.
The internal model of the Kalman filter is a linear adaptation of the minimal model of glucose kinetics (14), using feed-forward models to predict the plasma insulin and glucose rate of appearance, both inputs of the minimal model of glucose kinetics (8).
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