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Even though manufacturers provide clear directions on how to optimally use their kit and perform a certain assay, some laboratories adapt kits to their own protocols [ 12].
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Adapting kits are possible.
(a) Commercial IsoLink kit with 1 mL [99mTcO4]- (method 1), (b) modified IsoLink kit with 350 μL [99mTcO4]- (method 2) and (c) adapted kit with 100 μL [99mTcO4]- (method 3).
99mTc pertechnetate eluted with saline from a Drytec generator (GE Healthcare, Amersham, UK) was converted to [99mTc(CO)3(OH2)3]+ using an adapted kit from Isolink kit® (generously provided by Covidien, Petten, The Netherlands).
BoHV-1: Bovine herpesvirus type 1 gB: Glycoprotein B OPD: orthophenyldiamine OD N : Mean optical density of the negative control sera OD P : Mean optical density of the positive control sera OD S : Mean optical density of the sample sera Changes of the protocol to adapt the kit to reindeer are depicted in italic.
Negotiations are ongoing with several of these organizations to use the facility to produce locally adapted diagnostic kits for the African market.
We need kits to adapt what we are currently making to today's demands.
Middle-aged Paul and Kit must adapt to their roles as non-combatants, while Elinor's deeply held anti-war stance is challenged as her home town is bombarded.
Serum levels of osteocalcin, cortisol, ir-inhibin, urea and SAC were evaluated using commercially available kits, adapted for bovine serum, and namely N-MID® Osteocalcin Elisa (IDS, Frankfurt, Germany), Cortisol EIA kit® (Arbor Assays, Ann Arbor, MI, USA), Inhibin Alpha-Subunit (1 32) (Porcine) EIA kit® (Phoenix Pharmaceuticals, Inc., Karlsruhe, Germany), respectively.
Plasma glucose (Glucose RTU, BioMérieux, Marcy l′Etoile, France), triglycerides (PAP 150, BioMérieux), free fatty acids (NEFA C kit, Wako Chemicals, Neuss, Germany), and cholesterol (Cholesterol RTU, BioMérieux) concentrations were determined using commercial kits adapted to a microplate format, according to the recommendations of the manufacturer.
To facilitate this process, life-skills kits adapted to the Malagasy context have been revised and distributed in schools and youth clubs.
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