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All animals remained under isoflurane anaesthesia until they were euthanized by an intravenous injection of 1 ml phenobarbital (Esconarkon ad us. Vet., Streuli Pharma AG, Uznach, Switzerland) at the end of the experiment.
ad us.
Before anaesthesia, enrofloxacin (BAYTRIL 2.5% inj. ad us. vet., Bayer) was administered intravenously at the dose of 5 mg/kg.
+ atipamezol (1.0 mg/kg) (ANTISEDAN inj. ad us. vet., Pfizer Animal Health) administered intramuscularly after the surgery.
To prevent a bacterial infection, 10 mgkg−1 enrofloxacin (Baytril® 2.5% ad us. vet., Bayer, Leverkusen, Germany) was administered subcutaneously after the analgesic injections.
Analgesia in the post-operative period was achieved by administration of carprofen (RIMADYL inj. ad us. vet., Pfizer Animal Health) at the dose of 2 mg/kg/day for three days after the surgery.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com