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To acutely express recombinant genes, we used a Cre/loxP-mediated inducible expression system (Makino and Malinow 2011), in which Cre expression depends on 4-hydroxytamoxifen (4-OHT).
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To confirm that the repressive effect of RAS on autophagy in the HEK cells was not an artifact of long-term selection of the cells, we created new HRASG12V-expressing HEK- cells by acutely expressing HRASG12V in immortalized HEK cells that already stably expressed Tandem mCherry-GFP LC3.
We next acutely expressed constitutively active mutants of RAC1 and CDC42 in wild-type, p110α-, and p110β-knockout MEFs.
Valastyan et al. assessed the therapeutic potential of acutely expressing miR-31 in already-formed breast cancer metastases [ 142].
Acutely Expressed YFP-PrP∗ Undergoes RESET, Related to Figure 7 Shown is an NRK cell imaged for up to 15 hr after transient transfection with GFP-PrP∗.
Transcriptomic profiling of NKX3.1-negative prostate epithelial cells acutely expressing NKX3.1 revealed a rapid and complex response that is a near mirror image of the gene expression signature of human prostatic intraepithelial neoplasia (PIN).
The existence of alarm substances in communicating emotional stress via chemosensory cues is well-established in mammals [1], with animals exposed to odors secreted by acutely stressed conspecifics expressing neurobiological and behavioral changes consistent with increased arousal and threat-assessment [2] [4].
In the cortex and hippocampus, acutely exercised mutants expressed more BDNF than their sedentary counterparts.
Only acutely exercised mutants expressed significantly more BDNF than chronically exercised mutants, but not significantly more than their sedentary counterparts.
The fact that acutely run animals expressed more BDNF and TrkB than chronically run animals suggests that BDNF and TrkB expression change as the animals age and adapt to a regular, chronic exercise paradigm.
DA (100 µ m) was shown previously to increase the toxicity of the N-terminal fragment of mutated huntingtin (exon 1 of Htt with 123 polyglutamine repeats), expressed acutely after transfection (20).
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