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McGowin et al. [ 46] found that MG induce IL-6 in human A2EN and ShEN101 cells during acute secretion (48 h).
It must also be considered that in our study the collection of blood was subsequent to milk sampling, the first referring to the acute secretion and the latter to the previous 12 h secretion.
However, heterozygous mutant mice sensitized and challenged with ovalbumin, and then stimulated with an ATP aerosol to induce acute secretion, released only ~50% as much intracellular mucin as WT mice.
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Indeed, we now report that acute insulin secretion was substantially enhanced.
Glucose tolerance had further deteriorated and acute insulin secretion was weak and monophasic (ESM Fig. 6 a, b).
Acute insulin secretion (AIR) was calculated as the mean of 2- and 4-min insulin concentrations after glucose administration.
Conversely, numerous studies have shown that acute insulin secretion defects represent a powerful early predictor of diabetes progression.
β-Cell function was measured by HOMA-B and acute insulin secretion during the first 10 min after intravenous glucose tolerance test.
Clearly separate from the characteristic lack of acute insulin secretion in response to increase in glucose supply is the matter of total mass of β-cells.
Reduced glucose tolerance was further exacerbated at week 20 and acute insulin secretion was equally weak at week 20 (ESM Fig. 3b, c).
IPGTTs showed that the onset of hyperglycaemia was preceded by reduced glucose tolerance and significant delay in acute insulin secretion in young adult Aldh1b1tm1lacZ null mice.
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