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The acute preparation containing the whole hippocampus was obtained from PV-tdTomato mice according to a previously described protocol.
All of these studies either employed an invasive, acute preparation that can impact lymphatic function or non-invasive imaging of the lymphatics in the ear or tail where visual observations can be directly made.
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However, these investigations are limited to acute preparations or thin organotypic culture preparations due to the lack of a successful method that allows culturing of thick organotypic brain slices.
Acute preparations require extensive dissection causing tissue damage, which releases NO [41].
There are different terms in the literature describing NG2-like cells in acute preparations of wild type or different transgenic mouse lines: complex glial cells (e.g. [10]); GluR cells (e.g. [18]), OPCs (e.g. [19]), synantocytes [20], and polydendrocytes (e.g. [21]).
The functional analysis of the av6J, av3J and v2J mutants described in this paper was performed on cochlear cultures and acute preparations derived from the early postnatal cochlea because the hair cells are known to be particularly suitable for studying mechanotransduction at this stage of development [23], [24].
The majority of these studies involved acute preparations and described new processing algorithms.
Whole-cell voltage-activated K+ currents were recorded in IHCs from postnatal day (P) 18 P24 HC-BK−/− and the respective control mice (HC-BK-Ctr) using acute preparations of the organ of Corti as described previously (19).
Moreover, in agreement with Duport & Garthwaite (2005), the cell density was higher than in acute preparations: in the absence of LPS the cell density (per 146 × 146-μm area, including cells at the surface of the slice) was 26.8 ± 3.9 (five areas from four slices; P = 0.0001 compared with acute slices).
Since PTP has been observed mainly at synapses of acute preparations but not in cultured synapses, we developed an in vivo gene replacement strategy at the calyx of Held synapse, a large CNS model synapse at which PTP measurements are well established (see references above).
Methods: The study design used a nonblinded acute animal preparation.
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