The amount of IgG in 2 μl of each sera that actually bound to protein A/G beads was estimated by measuring the amount of bead-bound sera released by a low pH glycine elution buffer and measured using the BCA Protein Assay kit (Pierce Biotechnology Inc).
This was before we realized that we were actually bound for New Jersey, when we thought one of those lights was going to be our home.
Harvard Law School Library announces that a book in its collection that was believed to be bound in human skin is actually bound in sheepskin.
It is a persuasive document.To begin, the Little Sisters of the Poor and several dozen similar religious organisations are not actually bound by the contraceptive mandate.
The Times had been given hand-copied entries from Connolly's ledgers that laid out, penny for penny, the sums that were nominally going to fit out and furnish the courthouse, but were actually bound for the pockets of Boss Tweed and crew.
Therefore, the SLA Enforcer needs to know which traffic flows are actually bound to the same SLA.
I'm actually bound by the SFA judicial panel regulations and not able to explain the mitigating circumstances behind it.
To support our contention that the bacteria are actually bound to the pyrite particles, the samples were vigorously vortexed prior to imaging (see Figure 2c and 2d).
