Exact(6)
In T4 lysozyme, six mutations to Leu are analyzed within the wild-type background structure, and their Autobondrot score maps correctly predict whether or not their surroundings must shift significantly in the actual mutant structures; interactive examination of contacts for the conformations involved explains which clashes are relieved by the motions.
All other simulated mutants recovered configurations that mimic those observed in their corresponding actual mutant plants.
In a few cases, simulation results could not be compared to actual mutant configurations because such lines have not been reported yet (Table 5).
Since Li 2 is a monogenic dominant trait, it is possible that not one of these genes alone is the actual mutant allele causing the phenotype.
Therefore, analysis of an actual mutant gene library was employed in order to provide data with more direct relevance to practical library screening experiments.
One likely factor is skewed ratios of the actual mutant and WT proteins within a heterozygous sample, either in the expressed sample itself or ultimately what is captured on the ELISA plate, and this could occur through various mechanisms.
Similar(54)
These biases may influence studies such as drug resistance and vaccine resistance, which should reproduce the actual mutants in the field.
The simulated wer loss of function shows a hairy phenotype similar to that observed in actual wer mutants.
Already when using all but the diagonal and missing entries of the double-mutant fitness matrix in the dataset of St Onge et al. [10], we obtained an estimate relatively close to the actual measured single-mutant fitness vector, as compared to the conventional median estimate (Figure 1A).
First of all, it is not strictly necessary to have a reference genome sequence that exactly matches the actual parent of the mutant strains.
Finally, we confirmed the linearity between the actual mixed proportion of mutant and wild type plasmids and mA% detected by direct sequencing (Fig. 2b).
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