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Cytochrome P450 monooxygenase levels, and GST and NSE activity were determined using, respectively, tetramethylbenzidine, chlorodinitrobenzene, and α- and β-napthyl acetate substrates, as described in the WHO manual32.
Soil bacterial abundance and 16S rDNA transcriptional activity were determined using real-time PCR.
The optimal pH and temperature for KerP activity were determined using buffers of pH 5 12 at 30 90 °C.
Frequency components in this measure of population activity were determined using the Fast Fourier Transform.
HIF-1-dependent luciferase activities normalized to Renilla activity were determined using a Packard TopCount NXT (BioScience, Groningen, Netherlands).
The components of model polysiloxane coatings that alter enzyme activity were determined using commercial trypsin and transglutaminase (Figure 7).
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LDH activity was determined using a LDH Cytotoxicity Detection Kit (Takara, Ohtsu, Japan).
Twenty-four hours after transfection, luciferase activity was determined using the Dual-Luciferase RePromegaAssay System (Promega).
The peroxidase activity was determined using guaiacol as substrate.
Catalytic activity is determined using membrane electrode assembly (MEA) testing.
The HK activity was determined using the Hexokinase Assay Kit per manufacturer's protocol (Biomedical Research Service Center, SUNY, Buffalo, NY, USA).
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