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The effects of disease and drug on behavioural performance and regional brain activity were analysed using general linear models.
Samples for anti-Xa activity were analysed using a validated chromogenic assay kit (COAMATIC™ Heparin, Chromogenix, Instrumentation Laboratory Company, Lexington, MA, USA).
Meat-cooking methods, HCAs, B(a P, and total mutagenic activity were analysed using Computerized Heterocylic Amines Resource for Research in Epidemiology of Disease (CHARRED) (Sinha et al, 2005).
Courtship frequency and locomotor activity were analysed using two-factor ANOVA with selection regime as fixed factor crossed with random blocks.
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PC activity was analysed using a haemostasis laboratory analyser (BCS® XP; Siemens).
Creatine kinase activity was analysed using a commercially available kit (Pointe Scientific, Inc., Canton USA) with modifications to the protocol to allow for running the samples at 25°C.
The NF κB activity was analysed using the Dual-Luciferase RePromegaAssay System (Promega).
Enzyme activity was analysed using Sigma Plot Enzyme Kinetics Module (Systat Software, Richmond, CA).
Luciferase activity was analysed using the Steady-Glo Luciferase assay kit (Promega).
Cells were then harvested and luciferase activity was analysed using a manufactured kit (Promega UK Ltd) following the recommended guidelines.
Proteasomal activity was analysed using the bioluminescent Promega Proteasome-Glo™ assay (Promega) as recommended by the supplier.
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